Mir398 and plant stress responses pdf
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- miR398 and miR395 are involved in response to SO2 stress in Arabidopsis thaliana
- mir-398 microRNA precursor family
- Regulation of Non-coding RNAs in Heat Stress Responses of Plants
- Small RNAs in Plant Response to Abiotic Stress
miR398 and miR395 are involved in response to SO2 stress in Arabidopsis thaliana
Biotic and abiotic stresses affect plant development and production through alternation of the gene expression pattern. Gene expression itself is under the control of different regulators such as miRNAs and transcription factors TFs. Here, for the first time, seven conserved miRNAs, associated with drought, heat, salt and cadmium stresses were characterized in sunflower. The expression profiles of miRNAs and their targets were comparatively analyzed between leaves and roots of plants grown under the mentioned stress conditions. Gene ontology analysis of target genes revealed that they are involved in several important pathways such as auxin and ethylene signaling, RNA mediated silencing and DNA methylation processes. Gene regulatory network highlighted the existence of cross-talks between these stress-responsive miRNAs and the other stress responsive genes in sunflower. Based on network analysis, we suggest that some of these miRNAs in sunflower such as miR and miR may play critical roles in epigenetic responses to stress.
mir-398 microRNA precursor family
Recent findings have established that plants assign miRNAs as critical post-transcriptional regulators of gene expression in sequence-specific manner to respond to numerous abiotic stresses they face during their growth cycle. These small RNAs regulate gene expression via translational inhibition. Usually, stress induced miRNAs downregulate their target mRNAs, whereas, their downregulation leads to accumulation and function of positive regulators. In the past decade, investigations were mainly aimed to identify plant miRNAs, responsive to individual or multiple environmental factors, profiling their expression patterns and recognizing their roles in stress responses and tolerance. Altered expressions of miRNAs implicated in plant growth and development have been reported in several plant species subjected to abiotic stress conditions such as drought, salinity, extreme temperatures, nutrient deprivation, and heavy metals. These findings indicate that miRNAs may hold the key as potential targets for genetic manipulations to engineer abiotic stress tolerance in crop plants.
MicroRNAs play critical roles during plant development and in response to abiotic stresses. Send correspondence to. The fine-tuning role of miRNAs in addition to the regulatory role of transcription factors has shown that molecular events during development are tightly regulated. In addition, several miRNAs play crucial roles in the response to abiotic stress induced by drought, salinity, low temperatures, and metals such as aluminium. Interestingly, several miRNAs have overlapping roles with regard to development, stress responses, and nutrient homeostasis. Moreover, in response to the same abiotic stresses, different expression patterns for some conserved miRNA families among different plant species revealed different metabolic adjustments. The use of deep sequencing technologies for the characterisation of miRNA frequency and the identification of new miRNAs adds complexity to regulatory networks in plants.
Various studies have identified numerous miRNAs that were either up regulated or down regulated upon stress treatment. Our results showed that the regulation of miR in response to ABA and salt stress was more dynamic in plants than previously reported. We referred to this kind of regulation as dynamic regulation. In contrast, such dynamic regulation of miR under salt stress was completely absent in Arabidopsis, in which miR was steadily and unidirectionally suppressed. Interestingly, ABA treatment caused a deviate dynamic regulation of miR in Arabidopsis, showing an opposite response as compared to that in poplars.
Regulation of Non-coding RNAs in Heat Stress Responses of Plants
Heat stress is an important factor limiting plant growth, development, and productivity; thus, plants have evolved special adaptive mechanisms to cope with high-temperature stress. Recently developed advanced technologies, such as genome-wide transcriptomic analysis, have revealed that abundant ncRNAs are expressed under heat stress. Although this area of research is still in its infancy, an increasing number of several classes of regulatory ncRNA i. Finally, we briefly discuss future prospects concerning the ncRNA-related mechanisms of heat stress responses in plants. Abiotic stresses, such as heat, drought, salinity, and low temperature, seriously impact the growth and productivity of plants.
Sulfur dioxide SO2 is a common air pollutant that has adverse effects on plants. The activity of superoxide dismutase SOD , one of the major antioxidant enzymes, was enhanced with the increase in the CSD transcript level, suggesting an important role of miR in response to SO2-induced oxidative stress. Meanwhile, the expression of miR was increased, and the transcript levels of its target genes, ATP sulfurylases APS3 and APS4 and a low-affinity sulfate transporter SULTR2;1 , were decreased in Arabidopsis shoots, showing that miR played important roles in the regulation of sulfate assimilation and translocation during SO2 exposure. The content of glutathione GSH , an important sulfur-containing antioxidant, was enhanced with the changes in sulfur metabolism in Arabidopsis shoots under SO2 stress. These result
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Received: January 01, Published: ,. Citation: DOI:.
Small RNAs in Plant Response to Abiotic Stress
Sulfur dioxide SO 2 is a common air pollutant that has adverse effects on plants. The activity of superoxide dismutase SOD , one of the major antioxidant enzymes, was enhanced with the increase in the CSD transcript level, suggesting an important role of miR in response to SO 2 -induced oxidative stress. Meanwhile, the expression of miR was increased, and the transcript levels of its target genes, ATP sulfurylases APS3 and APS4 and a low-affinity sulfate transporter SULTR2;1 , were decreased in Arabidopsis shoots, showing that miR played important roles in the regulation of sulfate assimilation and translocation during SO 2 exposure. The content of glutathione GSH , an important sulfur-containing antioxidant, was enhanced with the changes in sulfur metabolism in Arabidopsis shoots under SO 2 stress.
Metrics details. Sequencing of 24 small RNA libraries produced From these, miRNAs were ascertained, of which mature miRNA evidence was obtained for and 36 were found to be differentially expressed after heat stress. PARE sequencing validated the targets of the conserved members of miRNA, miR and miR families as squamosa promoter-binding-like, homeobox leucine-zipper and transport inhibitor responsive proteins, respectively. Heat stress responsive miRNA targeted superoxide dismutases and an array of homeobox leucine-zipper proteins, F-box proteins and protein kinases.
Metrics details. Although diverse assays have been performed, systematic and detailed studies of miRNA expression and function during exposure to multiple environments in crops are limited. Here, we present such pioneering analysis in melon plants in response to seven biotic and abiotic stress conditions. Deep-sequencing and computational approaches have identified twenty-four known miRNAs whose expression was significantly altered under at least one stress condition, observing that down-regulation was preponderant. Additionally, miRNA function was characterized by high scale degradome assays and quantitative RNA measurements over the intended target mRNAs, providing mechanistic insight.
Among stress‐responsive miRNAs, miRNA (miR) is a miRNA proposed to be directly linked to the plant stress regulatory network and Overview of the role of miRNAs in plant stress responses Download PDF. back.