Pour plate and spread plate method pdf
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- Pour plate Method: Principle, Procedure, Uses, and (Dis) Advantages
- Colony Forming Unit Calculations Using Spread Plate Method
- Aseptic Laboratory Techniques: Plating Methods
Spread plate technique is the method of isolation and enumeration of microorganisms in a mixed culture and distributing it evenly. The technique makes it easier to quantify bacteria in a solution.
Pour plate Method: Principle, Procedure, Uses, and (Dis) Advantages
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Molten cooled agar approx. Microorganisms will grow both on the surface and within the medium. Colonies that grow within the medium generally are small in size and may be confluent; the few that grow on the agar surface are of the same size and appearance as those on a streak plate.
Each both large and small colony is carefully counted using magnifying colony counter if needed. The number of microorganisms present in the particular test sample is determined using the formula:. To insure a countable plate a series of dilutions should be plated. The pour plate method of counting bacteria is more precise than the streak plate method , but, on the average, it will give a lower count as heat sensitive microorganisms may die when they come contact with hot, molten agar medium.
It has the advantage of not requiring previously prepared plates, and is often used to assay bacterial contamination of food stuffs. Note: for the detail description regarding use of pipette, inoculation of sample, dilution technique etc, follow the reference 1. Pouring the molten agar medium Hold the bottle in the right hand; remove the cap with the little finger of the left hand. Flame the neck of the bottle. Flame the neck of the bottle and replace the cap.
Gently rotate the dish to mix the culture and the medium thoroughly and to ensure that the medium covers the plate evenly. Do not slip the agar over the edge of the petri dish. Allow the agar to completely gel without disturbing it, it will take approximately 10 minutes. Overview of Pour plate method and spread plate method Results:. After hours, count all the colonies again: note that the embedded colonies will be much smaller than those which happen to form on the surface.
A magnifying colony counter can aid in counting small embedded colonies. The sample is examined to detect the presence of gastrointestinal parasites or any other […]. Dear all I am excited to share with you all that, main authors of microbeonline.
X factor hemin and V factor nicotinamide-adenine-dinucleotide, NAD requirement test are done for the identification and differentiation of Haemophilus spp.
Haemophilus spp […]. Quite helpful…!!! M in fy level now…will b helpful to me? Can you please tell me , why there is white patches occcur in plate count agar even when no microbial growth white patches still form on the plates. Why this happen with plate count agar? During pour plate method, spreader colony found during total viable count then how to overcome this problem and which precautions take care during analysis. Does flaming the top of the agar jar add any benefit if performing testing in a laminar flow hood?
It seems that organisms would not be able to enter the bottle as the flow sweeps out of the hood. This site uses Akismet to reduce spam. Learn how your comment data is processed. Culture Media Tests microbes Difference Between. News Ticker. About Acharya Tankeshwar Articles. Hello, thank you for visiting my blog. I am Tankeshwar Acharya. Blogging is my passion. I am working as an Asst. Next Microbes with Characteristics Smell good and bad.
Lab Diagnosis of Parasitic Disease. Life of Bidur. April 2, Acharya Tankeshwar Life of Bidur 1. Would anaerobic bacteria grow in a pour plate as inoculated and incubated above? Why use ml sample for water analysis through membrane filter method.
Who authored the pour plate method please? How do I reference the method? Do you have any queries? Please leave me in the comments section below. I will be happy to read your comments and reply. Cancel reply.
Colony Forming Unit Calculations Using Spread Plate Method
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Molten cooled agar approx. Microorganisms will grow both on the surface and within the medium. Colonies that grow within the medium generally are small in size and may be confluent; the few that grow on the agar surface are of the same size and appearance as those on a streak plate. Each both large and small colony is carefully counted using magnifying colony counter if needed. The number of microorganisms present in the particular test sample is determined using the formula:.
Aseptic Laboratory Techniques: Plating Methods
Microorganisms are present on all inanimate surfaces creating ubiquitous sources of possible contamination in the laboratory. Experimental success relies on the ability of a scientist to sterilize work surfaces and equipment as well as prevent contact of sterile instruments and solutions with non-sterile surfaces. Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. Procedures described include 1 streak-plating bacterial cultures to isolate single colonies, 2 pour-plating and 3 spread-plating to enumerate viable bacterial colonies, 4 soft agar overlays to isolate phage and enumerate plaques, and 5 replica-plating to transfer cells from one plate to another in an identical spatial pattern.
For its higher productivity, R2A medium should be recommended for heterotrophic plate counts in natural mineral water. This is a preview of subscription content, access via your institution. Rent this article via DeepDyve. Cavalli-Sforza, L. Analisi statistica per medici e biologi.
Skip to main content. Search form Search. Spread plate method. Figure 2. The design method is based on the recent research of Shentu, Jiang and Hsu.
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